Journal: Oncotarget

Article Title: CXCR6-CXCL16 axis promotes prostate cancer by mediating cytoskeleton rearrangement via Ezrin activation and α v β 3 integrin clustering

doi: 10.18632/oncotarget.6944

Figure Lengend Snippet: PC3 and LNCaP cells cultured on cover slips were treated with 100ng/ml CXCL16 for 5 min with or without Calphostin C (100 nM) or Wortmannin (10 μM) pretreatment. Cells were stained with Rhodamine Phallodin and Alexa Fluor 488 conjugated Mouse anti-Ezrin (pY353) antibody. Images were captured using Olympus FluoView FV1000 confocal microscope with 60X oil immersion objective. Scale bar represents 20μm.

Article Snippet: After washing with wash buffer (Cytoskeleton Inc. Denver), cells were incubated for 40 minutes with 100nM Rhodamine Phallodin and 20μl Alexa Fluor 488 conjugated Mouse anti-Ezrin (pY353) (BD Biosciences).

Techniques: Cell Culture, Staining, Microscopy

Journal: Cells

Article Title: Blood and Sputum Eosinophils of COPD Patients Are Differently Polarized than in Asthma

doi: 10.3390/cells12121631

Figure Lengend Snippet: The comparison of CD125, CD193, CD62L, CD66b, CD14, CD11b expression on eosinophils in blood ( n = 10, 11, 14) and sputum ( n = 8, 9, 13) of control, asthma and COPD subjects. The data are presented as median (column) and individual data. Statistical significance between study groups was labeled as * for p -value ≤ 0.05; ** p -value ≤ 0.01; *** p -value ≤ 0.001.

Article Snippet: Cells were stained with antibodies against the surface binding molecules: CD45 (mouse anti-human APC-H7, cat. no. 641408), CD16 (mouse anti-human BV510, cat. no. 563830), CD125 (mouse anti-human BV421, cat. no. 743927), CD193 (mouse anti-human PE, cat. no. 558165), CD62L (mouse anti-human BV605, cat. no. 562719), CD66B (mouse anti-human PerCP-Cy5-5, cat. no. 562254), CD11B (rat anti-human APC, cat. no. 564985) and CD14 (mouse anti-human Alexa Fluor 488, cat. no. 557700) (BD Biosciences, San Jose, CA, USA) in BD Horizon Brilliant Stain Buffer (BD Biosciences, San Jose, CA, USA), and incubated for 20 min in the dark at room temperature.

Techniques: Expressing, Labeling

Journal: Cells

Article Title: Blood and Sputum Eosinophils of COPD Patients Are Differently Polarized than in Asthma

doi: 10.3390/cells12121631

Figure Lengend Snippet: The distribution of blood ( n = 10, 11, 14) ( A ) and sputum ( B ) ( n = 8, 9, 13) subpopulations of eosinophils defined by the expression of CD193 and CD125 in control, asthma and COPD groups. The data are presented as median (column), interquartile range (IQR) (whiskers) and individual data (points). Statistical significance between study groups was labeled as * for p -value ≤ 0.05; ** p -value ≤ 0.01; *** p -value ≤ 0.001; **** p -value ≤ 0.0001.

Article Snippet: Cells were stained with antibodies against the surface binding molecules: CD45 (mouse anti-human APC-H7, cat. no. 641408), CD16 (mouse anti-human BV510, cat. no. 563830), CD125 (mouse anti-human BV421, cat. no. 743927), CD193 (mouse anti-human PE, cat. no. 558165), CD62L (mouse anti-human BV605, cat. no. 562719), CD66B (mouse anti-human PerCP-Cy5-5, cat. no. 562254), CD11B (rat anti-human APC, cat. no. 564985) and CD14 (mouse anti-human Alexa Fluor 488, cat. no. 557700) (BD Biosciences, San Jose, CA, USA) in BD Horizon Brilliant Stain Buffer (BD Biosciences, San Jose, CA, USA), and incubated for 20 min in the dark at room temperature.

Techniques: Expressing, Labeling

Journal: PLoS ONE

Article Title: Epithelium-Intrinsic MicroRNAs Contribute to Mucosal Immune Homeostasis by Promoting M-Cell Maturation

doi: 10.1371/journal.pone.0150379

Figure Lengend Snippet: Primer sequence of q-PCR.

Article Snippet: The whole mount specimens were then stained overnight at 4°C with 1 μg/ml Alexa Fluor 488-conjugated anti-mouse GP2 (2F11C3; MBL).

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Epithelium-Intrinsic MicroRNAs Contribute to Mucosal Immune Homeostasis by Promoting M-Cell Maturation

doi: 10.1371/journal.pone.0150379

Figure Lengend Snippet: (A) H&E staining of small intestines VE region of Dicer ΔIEC and Dicer F/F . (B) H&E staining in PPs of Dicer ΔIEC and Dicer F/F . (C) The total number of follicles and surface area in Dicer ΔIEC and Dicer F/F . Data are means± SE (n = 3). * P < 0.05. (D) Whole mount immunostaining of PPs with anti-GP2 (red) and F-actin (green) analyzed using a confocal microscope. Scale bars: 100 μm (E) M cell number/mm 2 in FAE of each mouse strain. Data are means and SE. * P < 0.05.

Article Snippet: The whole mount specimens were then stained overnight at 4°C with 1 μg/ml Alexa Fluor 488-conjugated anti-mouse GP2 (2F11C3; MBL).

Techniques: Staining, Immunostaining, Microscopy

Journal: PLoS ONE

Article Title: Epithelium-Intrinsic MicroRNAs Contribute to Mucosal Immune Homeostasis by Promoting M-Cell Maturation

doi: 10.1371/journal.pone.0150379

Figure Lengend Snippet: (A) Flowchart of M cell maturation. (B) Q-PCR analysis was performed for Marcksl1 , SpiB , Ccl9 and Gp2 mRNA expression in Dicer ΔIEC FAE and Dicer F/F FAE. The relative expression levels of each gene to Gapdh are shown. Values represent the mean ± SD of three samples from different mice. * P < 0.05 ** P < 0.01.

Article Snippet: The whole mount specimens were then stained overnight at 4°C with 1 μg/ml Alexa Fluor 488-conjugated anti-mouse GP2 (2F11C3; MBL).

Techniques: Expressing